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Toehold Designer
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Domain type
Prokaryote
Eukaryote
Organism type
S. cerevisiae
K. pastoris
Hek cells
CHO cells
Customized
Trigger extraction method
Explicit trigger segment
Explicit trigger RNA
Trigger segment
Trigger RNA
N optimal trigger segments
Trigger segment size
Min trigger GC ratio
Max trigger GC ratio
Optimize trigger RNA codons for translation
Expression ORF
Show advanced options
Kozak location
In loop
After stem
Kozak sequence
Trigger binding location
Before stem
After stem
Stem size (To be opened by trigger)
Remain stem size (near loop unopened stem by trigger)
Loop size
Trigger binding site size (not including stem)
Kozak/RBS start position offset
RBS sequence
Linker sequence
Linker start position offset
Optimize linker codons for translation
5 Prime prefix sequence
N expression ORF prefix codons to include in optimization
Optimize expression ORF for translation
Optimize prefix expression ORF for lower defect
N prefix expression ORF codons to optimize for lower defect
Avoid pseudoknots
Perform reading frame and stop codons check
Avoid same nucleotide highly repeats
Minimum number of same nucleotide repeats to avoid
Perform uridine depletion
Detect epitopes in generated switch ORF
Detect organism similar off target RNA segments
N seeds
Temperature